Establishment of a rearing system of the extremotolerant tardigrade Ramazzottius varieornatus: a new model animal for astrobiology

Studies on the ability of multicellular organisms to tolerate specific environmental extremes are relatively rare compared to those of unicellular microorganisms in extreme environments. Tardigrades are extremotolerant animals that can enter an ametabolic dry state called anhydrobiosis and have high tolerance to a variety of extreme environmental conditions, particularly while in anhydrobiosis. Although tardigrades have been expected to be a potential model animal for astrobiological studies due to their excellent anhydrobiotic and extremotolerant abilities, few studies of tolerance with cultured tardigrades have been reported, possibly due to the absence of a model species that can be easily maintained under rearing conditions. We report the successful rearing of the herbivorous tardigrade, Ramazzottius varieornatus, by supplying the green alga Chlorella vulgaris as food. The life span was 35 +/- 16.4 d, deposited eggs required 5.7 +/- 1.1 d to hatch, and animals began to deposit eggs 9 d after hatching. The reared individuals of this species had an anhydrobiotic capacity throughout their life cycle in egg, juvenile, and adult stages. Furthermore, the reared adults in an anhydrobiotic state were tolerant of temperatures of 90 degrees C and -196 degrees C, and exposure to 99.8% acetonitrile or irradiation with 4000 Gy (4)He ions. Based on their life history traits and tolerance to extreme stresses, R. varieornatus may be a suitable model for astrobiological studies of multicellular organisms.     

Studies on the imaging characteristics of Yohkoh Soft X-ray Telescope: Optical aberration and scattering

In order to better understand the characteristics of Yohkoh Soft X-ray Telescope (SXT) mirror, we have analyzed the in-flight overexposed image (the starburst image) obtained during the solar flare observation. It has been revealed from our study that the intensity distribution inside the shadows shown in the scattering difference image contains little of the scattered component of the PSF and matches almost correctly the extension of the PSF core profile. Also it is found that the scattering wing of the SXT PSF is connected smoothly to the PSF core within the distance of about 100–200 arcsec from the peak. With numerical simulations we have shown that an increase in energy affects not only the level of scattering wing, but also both the shape and the absolute level of the PSF core. The results have revealed, however, that the energy dependence for the SXT PSF cannot be easily estimated with the data obtained from one filter alone, which implies that the data analysis using multiple filters will enable us to determine the absolute amount of scattered component as well as the energy dependence of the SXT PSF. Details on the analysis of starburst image and the results from numerical simulations will be introduced and discussed thoroughly.     

Use of sunlight for plant lighting in a bioregenerative life support system – Equivalent system mass calculations

Plant lighting is a critical issue for cost effectiveness of bioregenerative systems. A plant lighting system using sunlight has been investigated and compared to systems using electrical lighting. Co-generation of electricity and use of in situ resource utilization (ISRU) were also considered. The fixed part of equivalent system mass was found to be reduced by factors of from 3.1 to 3.9, according to the mission assumptions. The time-dependent part of equivalent system mass was reduced by a smaller value, of about 1.05. Cost effectiveness of bioregeneration has been compared to the cost of shipping food. Break-even times for different Lunar and Mars missions were generally in the order of 2–10 years, and were quite sensitive to the assumptions. There is significant scope for future refinement of these values, and work is ongoing.     

Characteristics of piezoelectric lead zirconate titanate multilayered detector bombarded with hypervelocity iron particles

A cosmic dust detector is currently being developed using a piezoelectric lead zirconate titanate (PZT) element. The characteristics of the multilayered detector (MD), which was composed of one hundred PZT disks, were investigated by bombarding it with hypervelocity iron particles supplied by a Van de Graaff accelerator. It was confirmed that there was a linear relationship between the signal amplitude observed from MD and the momentum of the particles. As compared with the single-layered detector (SD) that was composed of one PZT disk, it was found that the sensitivity of MD was ∼3 times higher than that of SD within the limits of the experimental conditions.     

The variation of equatorial spread-F occurrences observed by ionosondes at Thailand longitude sector

The equatorial spread-F (ESF) is a phenomenon of ionopheric irregularities which are mainly generated by the generalized Rayleigh–Taylor (R–T) instability mechanism in conjunction with the other physical mechanisms, originated at the bottom side of the F-layer in the equatorial region after sunset. It degrades the quality of signals that propagate through these irregularities, especially in the navigation satellite system, which requires the high integrity signals. In this work, we analyze the ESF statistics obtained from the FM/CW ionosonde stations over Thailand longitude sector. One is at Chumphon (10.72°N, 99.37°E, dip latitude 3.0°), located near the geomagnetic equator, and the other station is located at Chiangmai (18.76°N, 98.93°E, dip latitude 12.7°). Both stations are as part of the South-East Asia Low Latitude Ionospheric Network (SEALION) project. The ionograms are obtained at every 15 min from September 2004 to August 2005, which has the monthly mean of solar 10.7 cm flux (F10.7) from ∼80 to ∼110. In addition, we compare the diurnal patterns between the ESF occurrences and the variation of virtual height of the F-layer bottom side (h’F) of these two stations. The results show that the ESF occurrences at Chumphon stations are higher than Chiangmai station in all seasons. The high ESF occurrences of both stations mostly occur in equinoctial months corresponded with the rapid rising of the monthly mean h’F in the post-sunset. However, some inconsistent results are still observed, implying the role of other factors such as gravity waves and planetary waves to ESF occurrences.     

3-D magnetic field and current system in the heliosphere

In this paper a global system of the magnetic field and current from the interaction of the solar wind plasma and the interstellar medium is modeled using a 3-D MHD simulation. The terminal shock, the heliopause and the outer shock are clearly determined in our simulation. In the heliosheath the toroidal magnetic field is found to increase with the distance from the sun. The magnetic field increases rapidly in the upstream region of the heliosheath and becomes maximum between the terminal shock and the heliopause. Hence a shell-type magnetic wall is found to be formed in the heliosheath. Because of this magnetic wall the radially expanding solar wind plasma changes its direction tailward in all latitudes except the equatorial region. Only the equatorial disk-like plasma flow is found to extend to the heliopause through the weak magnetic-field region around the equator. Two kinds of global current loops which sustain the toroidal magnetic field in the heliosphere are found in our simulation.The influence of the 11-year solar cycle variation of the magnetic polarity is also examined. It is found that the polarity of the toroidal magnetic field in the heliosheath switches at every solar cycle change. Hence the heliosheath is found to consist of such magnetized plasma bubbles. The neutral sheets are found to extend between such magnetized plasma bubbles in the 3-D heliosheath in a complicated form. The magnetic-pressure effect on the heliosheath plasma structure is also examined.     

Development status of the high-speed reentry system-DASH

Despite huge amount of data collected by the previous interplanetary spacecraft and probes, the origin and evolution of the solar system still remains unveiled due to limited information they brought back. Thus, the Institute of Space and Astronautical Science (ISAS) of Japan has been given a commitment to pave the way to an asteroid sample return mission: the MUSES-C project. A key to success is considered the reentry with hyperbolic velocity, which has not ever been demonstrated as yet. With this as background, a demonstrator of atmospheric reentry system, DASH, has been designed to demonstrate the high-speed reentry technology as a GTO piggyback mission. The capsule, identical to that of the sample return mission, can experience the targeted level of thermal environment even from the GTO by tracing a specially designed reentry trajectory. After the purpose of the mission was outlined at the last IAF symposium, the final fitting tests have been conducted in the ISAS Sagamihara Campus involving the flight model hardware. Furthermore, a series of rehearsals for recovery have been already executed. The paper describes the current mission status of the project.     

Tolerance of anhydrobiotic eggs of the Tardigrade Ramazzottius varieornatus to extreme environments

Tardigrades are tiny (less than 1?mm in length) invertebrate animals that have the potential to survive travel to other planets because of their tolerance to extreme environmental conditions by means of a dry ametabolic state called anhydrobiosis. While the tolerance of adult tardigrades to extreme environments has been reported, there are few reports on the tolerance of their eggs. We examined the ability of hydrated and anhydrobiotic eggs of the tardigrade Ramazzottius varieornatus to hatch after exposure to ionizing irradiation (helium ions), extremely low and high temperatures, and high vacuum. We previously reported that there was a similar pattern of tolerance against ionizing radiation between hydrated and anhydrobiotic adults. In contrast, anhydrobiotic eggs (50% lethal dose; 1690 Gy) were substantially more radioresistant than hydrated ones (50% lethal dose; 509 Gy). Anhydrobiotic eggs also have a broader temperature resistance compared with hydrated ones. Over 70% of the anhydrobiotic eggs treated at either -196°C or +50°C hatched successfully, but all the hydrated eggs failed to hatch. After exposure to high-vacuum conditions (5.3×10(-4) Pa to 6.2×10(-5) Pa), the hatchability of the anhydrobiotic eggs was comparable to that of untreated control eggs.